2) Prepare 550 ml of 0.75M K2HPO4 buffer. The formula weight K2HPO4 is 174.176 g/mol.

2. We use the following instruments to measure >25ml we use graduated cylinders to measure 10 to 25ml we use 25ml glass pipettes to measure 5 to 10ml, we use 10ml glass pipettes to measure 1 to 5ml, we use 5ml glass pipettes to measure 200 ul to 1ml, we use the P1000 (blue pipetman) with large tips to measure 20 to 200ul, we use the P200 (bright yellow pipetman) with medium tips to measure 2 to 20ul, we use the P20 (light yellow pipetman) with medium tips to measure 0.2 to 2ul, we use the P2 (orange pipetman) with smallest tips