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28. Protein sequencing 2. Determine the sequence of a pep- tide consisting of 14 amino acids on the basis of the follow- ing data. Amino
28. Protein sequencing 2. Determine the sequence of a pep- tide consisting of 14 amino acids on the basis of the follow- ing data. Amino acid composition: (48,2L,F,G,I,K,M,T,W,Y) N-terminal analysis: S Carboxypeptidase digestion: L Trypsin digestion: (35,2L,F,I,M,T,W) (G,K,S,Y) Chymotrypsin digestion: (F,I,S) (G,K,L) (L,S) (M,T) (SW) (S,Y) N-terminal analysis of (F,1,S) peptide: S Cyanogen bromide treatment: (2S,F,G,I,K,L,M*,T,Y) (2S,L,W) M*, methionine detected as homoserine
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