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3. Create a recipe for 30.0mL of a phosphate buffered saline solution containing: 25mM Sodium Phosphate, pH7.4,140mMNaCl. You will be provided stock solutions of 0.500MNa2HPO4,0.500MNaH2PO4,

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3. Create a recipe for 30.0mL of a phosphate buffered saline solution containing: 25mM Sodium Phosphate, pH7.4,140mMNaCl. You will be provided stock solutions of 0.500MNa2HPO4,0.500MNaH2PO4, solid NaCl and water. Ignore the contribution of Na+from the phosphate buffers in your salt calculation. The pKa for HPO42 /H2PO4=6.86, and the molecular weight of NaCl=58.45g/mol. Show your work. mL0.500MNaH2PO4 gNaCl Fill to 30.0mL with distilled water

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