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3. Nitrite is commonly determined by a spectrophotometric procedure using the Griess reaction. The sample containing nitrite is reacted with sulfanilamide and N-(1-naphthyl) ethylenediamine to
3. Nitrite is commonly determined by a spectrophotometric procedure using the Griess reaction. The sample containing nitrite is reacted with sulfanilamide and N-(1-naphthyl) ethylenediamine to form a colored species that absorbs radiation at 550nm. Five-milliliter aliquots of the sample were pipetted into five 50.00 - mL volumetric flasks. Then, 0.00,2.00, 4.00,6.00, and 8.00mL of a standard solution containing 10.00uM nitrite were pipetted into each flask, and the color-forming reagents added. After dilution to volume, the absorbance for each of the five solutions was measured at 550nm. The absorbances were 0.139,0.299,0.486,0.689, and 0.865, respectively. Devise a spreadsheet to calculate the nitrite concentration in the original sample and its standard deviation
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