3. The AG' for the aldol cleavage of fructose-1,6-bisphosphate is +23.8 kJ/mol, indicating that this reaction favors the reactant. This is contrary to the observations made under cellular conditions. Calculate the actual free energy change (AG) observed under cellular conditions (see table below). Provide a qualitative description of the meaning of the calculated value (i.e. What does the value you calculate tell you?). Intermediate fructose-1,6-bisphosphate dihydroxyacetone phosphate glyceraldehyde-3-phosphate Concentration 35 M 150 MM 20 M 3a) Hydrolysis of adenosine triphosphate (ATP) is highly favorable under standard conditions. Provide and discuss one reason for this observation. 3b) In glycolysis, Step 6: The Oxidation of Glyceraldehyde-3-Phosphate is thermodynamically unfavorable under standard conditions. To "pull" the reaction forward, Step 6 is coupled with Step 7: The 1st Production of ATP. What is the meaning of the term coupled? Calculate the overall AG' for Steps 6 and 7. 3. The AG' for the aldol cleavage of fructose-1,6-bisphosphate is +23.8 kJ/mol, indicating that this reaction favors the reactant. This is contrary to the observations made under cellular conditions. Calculate the actual free energy change (AG) observed under cellular conditions (see table below). Provide a qualitative description of the meaning of the calculated value (i.e. What does the value you calculate tell you?). Intermediate fructose-1,6-bisphosphate dihydroxyacetone phosphate glyceraldehyde-3-phosphate Concentration 35 M 150 MM 20 M 3a) Hydrolysis of adenosine triphosphate (ATP) is highly favorable under standard conditions. Provide and discuss one reason for this observation. 3b) In glycolysis, Step 6: The Oxidation of Glyceraldehyde-3-Phosphate is thermodynamically unfavorable under standard conditions. To "pull" the reaction forward, Step 6 is coupled with Step 7: The 1st Production of ATP. What is the meaning of the term coupled? Calculate the overall AG' for Steps 6 and 7