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** 3) You are studying the kinetics of an enzyme with a molecular weight of 29.6 kDa. You conduct an experiment where you measure the
** 3) You are studying the kinetics of an enzyme with a molecular weight of 29.6 kDa. You conduct an experiment where you measure the amount of product produced in 1 minute in a 10 ml solution containing 10-9 g of the enzyme. In these experiments, the concentration of the substrate is in vast excess of the enzyme and does not change significantly during the course of the experiment. The results are shown below: [Substrate] (UM) 1 3 5 10 30 50 Product produced (nanomoles) 0.11 0.25 0.34 0.45 0.58 0.61 (a) Plot Michaelis-Menten and Lineweaver-Burk plots for these data. Does the enzyme appear to obey Michaelis-Menten kinetics? If so, what is the value of Km? (b) What is the value of Vmax ? (c) What is the turnover number (kcat) of this enzyme under these experimental conditions
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