3.14. a. H. H. Weetall and N. B. Havewala report the following data for the production of dextrose from corn starch using both soluble and immobilized (azo-glass beads) glucoamylase in a fully agitated CSTR system. 1. Soluble data: T=60C,[S0]=168mgstarch/ml,[E0]=11,600 units, volume =1000ml. 2. Immobilized data: T=60C,[S0]=336mg starch/ml, [E0]=46,400 units initially, immobilized, volume =1000ml. Determine the maximum reaction velocity, Vm(mg/mlmin - unit of enzyme) and the saturation constant, KM(mg/ml). b. The same authors studied the effect of temperature on the maximum rate of the hydrolysis of corn starch by glucoamylase. The results are tabulated next. Determine the activation energy ( Ecal/g mole) for the soluble and immobilized enzyme reaction. c. Using these results, determine if immobilized enzyme is diffusion limited. [Courtesy of A. E. Humphrey from "Collected Coursework Problems in Biochemical Engineering" compiled by H. W. Blanch for 1977 Am. Soc. Eng. Educ. Summer School.]