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3,4-Benzopyrene is a potent carcinogen often found in polluted air. A sensitive method for the determination of the carcinogen is to measure its fluorescence in
3,4-Benzopyrene is a potent carcinogen often found in polluted air. A sensitive method for the determination of the carcinogen is to measure its fluorescence in sulfuric acid solution. The excitation wavelength is 520 nm and the emission wavelength is 548 nm. Ten liters of air were drawn through 10.00 mL of dilute sulfuric solution. One milliliter of this sulfuric acid solution gave a reading of 33.3 in a fluorometer. Two different standard solutions of 3,4-benzopyrene containing 0.750 ug and 1.25 ug per 10.00 mL of sulfuric acid gave readings of 24.5 and 38.6, respectively, when 1.00 mL was placed in the same cell used for the unknown. A blank sample with no 3,4-benzopyrene gave a reading of 3.5. Calculate the weight of 3,4- benzopyrene in 1 liter of air
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