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( 4 0 points ) Component I in an aqueous media is purified using a chromatography column in length 2 . 5 c m in

(40 points) Component I in an aqueous media is purified using a chromatography column in
length 2.5cm in diameter) packed with 75m diameter porous particles with a bed porosity of 0.38.
The partition coefficient for I is 0.5. The retention time and base width data obtained for three different
linear velocities of the eluent are as follows:
(a) Determine the constants of the Van Deemter Equation and plot the height of theoretical plate as a
function of linear velocity. Determine the optimum linear velocity and minimum height of
theoretical plate graphically and compare it to the result obtained from Example 4-3.
(b) Calculate the number of theoretical plates for a linear velocity of 2cmmin.
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