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4. JYou have an oligo peptide with an unknown amino acid sequence and perform the Following experiments: Step1: Treatment with trypsin yields three fragments with
4. JYou have an oligo peptide with an unknown amino acid sequence and perform the Following experiments: Step1: Treatment with trypsin yields three fragments with sequences : GWA , HGK , VYDTR Step2: Treatment with chymotrypsin givesthree fragments with sequences: A,HGKVY ,DTRGW (a) What is the primary amino acid sequence of the peptide? (b) Why is Step 1 alone not sufficient to unambiguously determine the primary sequence? (c) Suppose your lab was out of chymotrypsin and chymotrypsin is on back order due to supply Chain issues. What alternative protease(s) could you use to unambiguously determine the Peptide sequence
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