4. Prepare 15 ml of TSB media containing 25ug/ml tetracycline from a 100 mg/ml tetracyclinestock solution.

4. Dilutions can be easily calculated as: C1V1=C2V2 Where, C1 and C2 are the concentrations of solution 1 and solution 2 , respectively And V1 and V2 are the volumes of solution 1 and solution 2 , respectively Note that C can be molarity (M) or percent (\%), but the units MUST be the same for both solutions. Note that for the volume the units ( vsml, for example) must also be the same for both solutions Example: Make 11 of 50mM Tris buffer from a 1M Tris buffer stock solution. C1=50mMV1=1l=1000mlC2=1M V2 is unknown =x (50mM)(1000ml)=(1000mM)(x) x=(50mM)(1000ml)/(1000mM)=50ml so add 50ml of the 1M Tris solution to 950ml of water 4. Dilutions can be easily calculated as: C1V1=C2V2 Where, C1 and C2 are the concentrations of solution 1 and solution 2 , respectively And V1 and V2 are the volumes of solution 1 and solution 2 , respectively Note that C can be molarity (M) or percent (\%), but the units MUST be the same for both solutions. Note that for the volume the units ( vsml, for example) must also be the same for both solutions Example: Make 11 of 50mM Tris buffer from a 1M Tris buffer stock solution. C1=50mMV1=1l=1000mlC2=1M V2 is unknown =x (50mM)(1000ml)=(1000mM)(x) x=(50mM)(1000ml)/(1000mM)=50ml so add 50ml of the 1M Tris solution to 950ml of water