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5. (10 pts) Bacillus subtilis growing on methanol has a maximum growth yield coefficient of 0.52 g X/g S. The heat of combustion of cells

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5. (10 pts) Bacillus subtilis growing on methanol has a maximum growth yield coefficient of 0.52 g X/g S. The heat of combustion of cells is 5.92 kcal/g cells and for combusting the substrate (AHC) is 7.2 kcal/g-methanol. The cells are growing in the exponential growth phase in a 3.5 L chemostat reactor with a steady state cell concentration of [X] = 12.8 g/L and a feed concentration of 28.3 g/L methanol, at the maximum specific growth rate, Umax, of 0.77 h 1. A. Determine the metabolic heat generated by the cells per unit mass of methanol consumption, Ys/s (in kcal/g S). {Hint: see class notes} B. At what rate must heat be removed from the reactor to maintain an isothermal reactor (in kcal/h)

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