5. (10 pts) The value of Km and rmax for an enzyme are 0.004 kmol/m and 10 kmol/ms. It was immobilized to the surface of acrylamide-based polymers that can be assumed to have spherical shape (diameter = 1 mm). The effectiveness of the immobilized enzyme was found to be 70% of the free enzyme when the concentration of the substrate was 0.5 kmol/m. The reaction was carried out in a stirred reactor with an agitation speed of 50 rpm. a. (5 pts) Estimate the concentration of the substrate at the surface of the immobilized enzyme. b. (5 pts) Estimate ksa