5. Immobilised cells of a genetically improved strain of Brevibacterium lactofermentum are used to convert glucose to glutamic acid for production of MSG (monosodium glutamate). The immobilised cells are unable to grow, but metabolise glucose according to the equation: C6H12O6+1.5O2+NH3C5H9O4N+CO2+3H2O A feed stream of 4% glucose in water enters a 25000L reactor at 25C at a flow rate of 2000kg/h. A gaseous mixture of 12%NH3 in air is sparged into the reactor at 1 atmospheric pressure and 15C at a flow rate of 4 vessel volume per min. The product stream from the reactor contains residual sugar at a concentration of 0.5%. (a) Estimate the cooling requirements. (15 marks) (b) What will be the temperature increase in the fermentation if no cooling is provided? (5 marks) Assume temperatures of product, reactor and off gases equal to 25C. Standard heat of combustion for the compounds (hc) at 25C and atmospheric pressure are: for glucose =2805kJ/gmo1; for ammonia =382.6kJ/gmol; for oxygen =0; for glutamic acid =2244.1kJ/gmol. Consider specific heat of all components mixture and water equal to 4.18kJ/(kgK)