5) Reversible Ligand Binding The protein calcineurin binds to the protein calmodulin with an association rate of 8.9103M1s1 and an overall dissociation constant, KD, of 10nM. Calculate the dissociation rate, kd, including appropriate units. (2 points total) B) The E. coli nickel-binding protein binds to its ligand, Ni+2, with a KD of 100nM. Calculate the Ni+2 concentration when the fraction of binding sites occupied by the ligand () is 0.95