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5. Two different solutions, A and B, are separated by a semipermeable membrane. Assume the membrane has a MWCO (molecular-weight cut-off) of 100,000 Da (Dalton

5. Two different solutions, A and B, are separated by a semipermeable membrane. Assume the membrane has a MWCO (molecular-weight cut-off) of 100,000 Da (Dalton which is the mass of one molecule and is numerically equivalent to the molecular weight, g/mol). Solution A consists of albumen (80 g/L; MW=69,000 g/mol) and fibrinogen (40 g/L; MW=340,000 g/mol). Solution B consists of albumen (10 g/L), globulins (50 g/L; MW=150,000), and fibrinogen (20 g/L). Assume the solvents for both solutions are water. If the hydrodynamic pressure of solution A is 700 mmHg, (A) determine the hydrodynamic pressure of solution B so that there is a net-zero flow of water across the membrane. (B) If the membrane MWCO is lowered to 50 kDa, determine the hydrodynamic pressure of solution B to maintain a net-zero flow of water across the membrane (assume the hydrodynamic pressure of A is still the same). Assume for both cases that the temperature of the entire system is at 37oC. (Ans: A=705.3 mmHg)

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