5. You need to make 10 tubes of culture media containing 10 ml of media per tube. The media is MS with GA you are using Sigma's Product # G7645 as your source of GA. How many ul of GA stock do you add in order to make the total batch of media? MW Solvent LIM for 1 mg/L. Stock Solution PGR Sigma Product # Auxins 2,4-D D7299 2,4-D (Na- salt) D6679 IAA 12886 IAA (Na-salt) 15148 IBA 15386 IBA (K-salt) 17512 Cytokinins BA B3408 BA-HCI B5920 Kinetin K0753 Miscellaneous PGRS ABA A1049 GA3 G7645 GA (K-salt) G1025 221 243 175.2 197.2 23.2 241.3 4.53 4.12 5.71 5.07 4.90 Water EtOH/1N NaOH Water EtOH/1N NAOH Water 4.14 225.3 261.7 215.5 4.44 3.82 4.65 1N NaOH Water IN NaOH 264.3 346.4 384.5 3.78 2.89 2.60 14 NaOH ETOH Water MS Media (1L liquid media) 4.4 g MS Basal Mix (Sigma 5519) 30 g Sucrose Adjust pH to 5.7 +0.1 before autoclaving For solidified media add 3.0 g of phytagel after pH adjustment 5. You need to make 10 tubes of culture media containing 10 ml of media per tube. The media is MS with GA you are using Sigma's Product # G7645 as your source of GA. How many ul of GA stock do you add in order to make the total batch of media? MW Solvent LIM for 1 mg/L. Stock Solution PGR Sigma Product # Auxins 2,4-D D7299 2,4-D (Na- salt) D6679 IAA 12886 IAA (Na-salt) 15148 IBA 15386 IBA (K-salt) 17512 Cytokinins BA B3408 BA-HCI B5920 Kinetin K0753 Miscellaneous PGRS ABA A1049 GA3 G7645 GA (K-salt) G1025 221 243 175.2 197.2 23.2 241.3 4.53 4.12 5.71 5.07 4.90 Water EtOH/1N NaOH Water EtOH/1N NAOH Water 4.14 225.3 261.7 215.5 4.44 3.82 4.65 1N NaOH Water IN NaOH 264.3 346.4 384.5 3.78 2.89 2.60 14 NaOH ETOH Water MS Media (1L liquid media) 4.4 g MS Basal Mix (Sigma 5519) 30 g Sucrose Adjust pH to 5.7 +0.1 before autoclaving For solidified media add 3.0 g of phytagel after pH adjustment