Question
5A). Tris buffer has a pKa of 8.07. You are asked to make 70 ml of 1.5 M Tris-HCl buffer at a pH of 8.8.
5A). Tris buffer has a pKa of 8.07. You are asked to make 70 ml of 1.5 M Tris-HCl buffer at a pH of 8.8. If Tris base (MW = 121.14) and Tris-HCl (MW=157.6), how many grams of each would you need to weigh out?
5B) Say you made the above buffer (70 ml of 1.5 M Tris-HCl, pH of 8.8) using only Tris base and then titrating with concentrated HCl (12.1 M), how many grams of Tris base and mL of 12.1 M HCL would be required?
5C) What about if you needed 70 ml of 1.5 M Tris-HCl buffer at a pH of 6.8? What is the amount of Tris base and 12.1 M HCl (in mL) would be needed to achieve a pH of 6.8?
Need help with 5A, 5B, and 5C, will upvote answer
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