7.15 Estimation of the Number of Theoretical Plates for Ion Exchange Chromatography of a Protein Following a Langmuir Isotherm A 20 cm long ion exchange column is loaded with a protein solution at a concentration of 3 mg/ml and is eluted isocratically at a superficial velocity of 30 cm/h. The void fraction is 0.3. The protein has a Keq (Langmuir isotherm) of 1.0 ml/mg, and the columns resin is saturated with the proteinat a resin-phase concentration of 10 mg/ml. If the protein peak has a standard deviation of 4.0 min, estimate the number of theoretical plates in the column.322 // BIOSePARATIONS SCIeNCe AND eNGINeeRING