8. (10 pts) An enzyme reaction is being made into a continuous process using immobilization. Data have been collected on the unimmobilized and uninhibited system using 100% active enzyme, and are shown in an Eadie-Hofstee plot at right (the equation of the line is shown on the plot). Beads containing enzyme are placed in a continuous flow reactor where substrate enters at [S]0=Sb=0.275M. Immobilized enzyme reactor parameters: totalbeadsurfaceareainthepackedcolumn%activeenzymeReactorvolumeReactionMechanism:effectivesubstratediffusivity,Ds,eEncapsulatingmembranethickness,680cm276mol%3.3LS+E1ES2E+P5.7106cm2/s11.7m(1.17105m) kinetic rate constants (k1,k1,k2) are NOT given, nor is there any information about porosity or tortuosity. Another challenge that your co-worker just reported is that the feed contains a non-competitive inhibitor, which cannot be removed from the substrate entering the reactor. The inhibitor, [1]=0.0105M, has dissociation constant, Ki=0.027M. Accounting for the inhibitor in the feed, evaluate whether the immobilized enzyme reaction is reaction- or diffusion- limited, and determine the rate of product formation (dP/dt) in mol/L-min that you would expect for the given [S] in the immobilized enzyme reactor