8. In the reaction below, the standard free energy of hydrolysis is given as - 76.3 kJ/mol. The reaction occurs at room temperature and pH of 7.0. The concentration of 1, 3-bis-phosphoglycerate is 47 M, phosphate is 5 M and 3-phosphoglycerate is 25 M. What is the total free energy change for this equation? If you make any approximations or assumptions, please mention below. Given R = 8.314 J/K-mol. (6 points) 1, 3-bis-phosphoglycerate + H2O 3-phosphoglycerate + Pi + H