8. Tris- HCl in solution will produce a pH of approximately 4.7 and Tris-Base in solution will produce a pH of approximately 10.4. Which one has the better buffering capacity and why? 9. Sucrose density gradient ultracentrifugation is a powerful technique for fractionating macromolecules like DNA, RNA, and proteins. Some protocols using sucrose gradients mention the following: 10mL sucrose gradients 1015%(w/v) in 10mM HEPES buffer, 150mMKCl and 5mMMgCl. How would you prepare this sucrose gradient? 10. Almost every biochemistry laboratory runs some kind of gel electrophoresis, either for DNA, RNA or proteins. Some buffers needed to run the gel electrophoresis has three or four components. Instead of listing the molar concentration of each chemical in the solution, a stock concentrated solution might be labeled i.e I0X SDS-PAGE Running Buffer. This tells the researcher that the stock solution is ten times more concentrated. Assume you need 50ml of 1X Running Buffer to perform an electrophoresis. How would you prepare the 1X Buffer from a 10X Stock Solution