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8. You have stock solutions of primers AluPV92 Forward and AluPV92 Reverse at 100M concentration. You need to make a PCR Master mix cocktail using

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8. You have stock solutions of primers AluPV92 Forward and AluPV92 Reverse at 100M concentration. You need to make a PCR Master mix cocktail using your 2X Master Mix and these primer stocks. The primers in your PCR Master mix cocktail should have a final (working) concentration of 0.2M. You want to make 200L of PCR Master mix cocktail (enough for four (4) 50L reactions using 20L of template per reaction) If you use the primers at their stock concentration of 100M, you would need to add 0.4L of each primer to the cocktail. It is very difficult to accurately pipette 0.4L. A better plan would be to dilute your primer stock solutions to a lower concentration so you can add a higher volume to your PCR Master Mix to reach the desired final concentration of 0.2M. After careful thought, you have decided to to dilute the 100M stock primers to a less concentrated stock solution before adding them to your cocktail. Remember, you cannot pipette less than 1L accurately, and it is best to adjust your stock solutions so that you will be able to pipette 2L or more. a. Dilute the 100M stocks of primers to 10M concentration. i. Make 100L of the 10M primer solutions. ii. Show that calculation and describe how you would make those new stock solutions

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