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9) Four litres of 0.4M 3-(N-morpholino) propane sulfonic acid (MOPS) buffer of pH 7.5 are required. Available in the laboratory are 10M HCI and
9) Four litres of 0.4M 3-(N-morpholino) propane sulfonic acid (MOPS) buffer of pH 7.5 are required. Available in the laboratory are 10M HCI and 10M NaOH and the zwitterionic form of MOPS (M.Wt=210.3). The pKa for the MOPS zwitter ion is 7.2. Describe, showing the relevant calculations, the procedure for preparing the buffer 10) If 10ml of 10M NaOH was added to 1 litre of the above buffer (question 9) what would be the resulting pH? 11) What would be the resulting pH if 10ml of 10M NaOH were added to 1 litre of pure water?
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