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A 100mL solution of 0.1M alanine at pH1.72 was titrated with 2MNaOH solution. The pH was monitored and the results were plotted on a graph,

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A 100mL solution of 0.1M alanine at pH1.72 was titrated with 2MNaOH solution. The pH was monitored and the results were plotted on a graph, as shown below. The key points in the titration are designated I to V. Identify the appropriate key point in the titration where the average net charge of alanine is 1

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