A 2093 liters protein solution of 30 g/L needs to be concentrated to 200 g/L. Also, the initial salt concentration must be reduced one hundredfold. The relationship between the permeation rate and the bulk protein concentration has been determined experimentally as follows: = 500(270/) where Q = permeation rate (L/h), Cb = bulk protein concentration (g/L) Usually, it starts from concentration, diafiltration and then concentration, to reduce the total operation time.

a) Determine the operation batch volume that will give the minimum time for diafiltration. b) Then determine the times required for (1) concentration to the volume at which diafiltration is to be carried out, (2) diafiltration, and (3) concentration to the final concentration desired

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