Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

A 5.00-mL sample of blood was treated with trichloroacidic acid to precipitate proteins. After centrifugation, the resulting solution was brought to a pH of 3

A 5.00-mL sample of blood was treated with trichloroacidic acid to precipitate proteins. After centrifugation, the resulting solution was brought to a pH of 3 and was extracted with two 5.00-mL portions of methyl isobutyl ketone containing the organic lead complexing agent APCD. The extract was aspirated directly into an air-acetylene flame yielding an absorbance of 0.577 at 283.3 nm. A five-milliliter aliquot of an aqueous standard solution containing 0.213 g/mL Pb was treated in the same way and yielded absorbance of 0.357. Calculate the concentration Pb (in g/mL) in the sample assuming that Beers law is followed.

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Introduction To Chemical Engineering Thermodynamics

Authors: H.C. Smith, J.M. & Van Ness

2nd Edition

0070586993, 978-0070586994

More Books

Students also viewed these Chemical Engineering questions

Question

=+2. What a terrible tragedy you have suffered.

Answered: 1 week ago