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A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: Vo (M/min) (without inhibitor) Vo
A biochemist wants to determine the effect of an inhibitor on a certain enzyme. The data are shown below: Vo (M/min) (without inhibitor) Vo (uM/min) (with inhibitor) [Substrate] (HM) 10 13.2 7.9 20 20.9 12.2 40 28.4 17.2 80 36.6 22.1 120 40.2 24.2 o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the absence of inhibitor. Vmax = + M/min Km = * M o Using linear regression analysis, determine the values of Vmax and Km of the enzyme in the presence of inhibitor. Vmax = * M/min Km = + M o What is the type of inhibition involved?
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