A colon cancer cell line was treated by decitabine (DAC) and vitamin C (VC). The concentration of DAC was always 1nM. There are three different concentrations of VC (VC1 is designated as a control). We would like to know whether the different treatments have different effects on gene expression. In addition, we also want to know if treat the cells for a longer time would result in more gene expression. So, we treated the cells for two durations: 12 hours and 24 hours. Each treatment had 4 biological repeats. Reactivation of an epigenetically silenced gene was measured by qPCR. Delta CT (the larger delta CT means the more gene expression) was reported in the table that summarized the results.