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A dalton is another name for atomic mass unit. For example, a protein with a mass of 1 5 k d means that the MW
A dalton is another name for atomic mass unit. For example, a protein with a mass of means that the MW is
tabletablemicromolartableAmount hydrolyzednanomoles
a Plot versus S for these data. Does penicillinase appear to obey MichaelisMenten kinetics? If so what is the value of
b What is the value of Indicate on the plot how you determined and
c Write the MichaelisMenten equation and show the derivation of the LineweaverBurke equation.
d Plot versus
e Determine and using the LineweaverBurke plot. Indicate on the plot how you determined and
f Assume there is one active site per enzyme molecule. What is the turnover number of penicillinase under these experimental conditions?
g How much time does it take for the penicillinase to hydrolyze one penicillin molecule?
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