Question
A fermentation slurry containing Bacillus stearothermophilus cells and produced -amylase protein is filtered using a continuous rotary vacuum filter. Slurry is fed to the filter
A fermentation slurry containing Bacillus stearothermophilus cells and produced -amylase protein is filtered using a continuous rotary vacuum filter. Slurry is fed to the filter at a rate of 218 kg/h; 1 kg slurry contains 23 g cell solids. To improve filtration rates, particles of diatomaceous earth filter aid are added at a rate of 14 kg/h. The concentration of -amylase protein in the slurry is 0.13 % by weight. Liquid filtrate is collected at a rate of 196.2 kg/h. The concentration of -amylase protein in the filtrate is 0.1235 % (w/w). Filter cake containing cells and filter aid is removed continuously from the filter cloth. Calculate different shares and streams (per hour) and answer questions. You can find help from example 4.3 in text book.
1. How much water is in the input stream (kg/h)?
2. What is the total input feed mass flow (kg/h)?
3. How much water is flowing in the filtrate stream (kg/h) ?
4. How much cells are removed with filter cake (kg/h) ?
5. How much is the filter cake output stream (kg/h) ?
6. How much -amylase is lost with the filter cake (kg/h) ?
7. How much water is removed with the filter cake (kg/h) ?
8. Output filtercake water content-% is:
9. Output filtercake -amylase content-% is:
10. Output filtercake cell content-% is:
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