A membrane having a porosity of 0.3, average pore size of 0.04 m, average pore tortuosity of 1.1, surface area of 2 cm2 and thickness of 0.1 mm separates two water-filled, well-mixed chambers one having a volume of 10 ml and the other having a volume of 20 ml. The content of the 10 ml chamber was replaced with 10 ml of 1 mg/ml insulin solution at t = 0. Determine if insulin is hindered by the pores of the membrane and calculate the insulin concentration in the other chamber after 5 hours. Assume that there is no convective flow of solvent through the membrane. This experiment was carried out at 20 degrees centigrade. MW of insulin = 5800 Da