A Mesophilic bacterium A utilizes glucose to produce an intracellular protein B and an extracellular byproduct C. The aerobic bacterium is metabolically active in the pH range of 6.9 7.2. A laboratory level study indicates 80% of the substrate conversion to product B in 72 hours. The activity of the protein is reported to decrease in the presence of non-polar organic solvents. The molecular weight of protein B is 65 kDa. Protein B is used as starting material for therapeutic applications. Design a process following RIPP scheme to purify the protein B and justify your design