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A pathway for the fermentative production of the monoterpene limonene (C 10 H 16 ) with the following stoichiometry was designed : 3 Glucose +

A pathway for the fermentative production of the monoterpene limonene (C10H16) with the following stoichiometry was designed :

3 Glucose + 8 NAD limonene + 8 CO2 + 8 NADH + 2 H2O

MW (Glucose, C6H12O6): 180 g/mol

MW (Limonene): 136 g/mol

What is the stoichiometric yield (in g product/ g substrate) ?

What percentage of the maximum theoretical (thermodynamic) yield is reached with this stoichiometry ?

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