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A peptide was treated separately with trypsin and chymotrypsin then sequenced by Edman degradation. The following fragments were obtained: Trypsin Digest - MQYVWR - WAIK

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A peptide was treated separately with trypsin and chymotrypsin then sequenced by Edman degradation. The following fragments were obtained: Trypsin Digest - MQYVWR - WAIK - CTR - HLFK - TLR Chymotrypsin Digest - KCTR - AIKMQY - VW - TLRW - RHLF A mass spectrum was obtained for this peptide in positive ion mode (meaning only positively charged ions will be detected). What is the charge on the peptide for the peak at m/z=528.44 ? Answer should be a whole number and include the sign of the charge (eg. +32). A

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