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A rule of thumb for reversed -phase isocratic separation predicts a retention time decrease of 1 - 2% for each 1 C column temperature increase.

A rule of thumb for reversed -phase isocratic separation predicts a retention time decrease of 1 - 2% for each 1 C column temperature increase. As the column temperature rises, the mobile phase viscosity decreases, thereby the flow resistance in the column, and the system backpressure as well

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