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7. Protein Blue dextran A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on

7. A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on a Sephadex G-200 column. The elution volume (Ve) for each protein is indicated in the table below. Estimate the molecular mass of the unknown protein. Protein Blue dextran Mr 1000 kDa Ve (mL) 85.00 lysozyme Chymotrypsinogen ovalbumin Serum albumin 14 kDa 25 kDa 45 kDa 65 kDa 200.00 190.00 170.00 150.00 aldolase urease ferritin 150 kDa 500 kDa 700 kDa 28 kDa 125 90.00 92.00 160.00 130.00 ovomucoid Unknown 8.

7. Protein Blue dextran A series of proteins with known molecular mass and an enzyme of unknown molecular mass are separated by chromatography on a Sephadex G-200 column. The elution volume (Ve) for each protein is indicated in the table below. Estimate the molecular mass of the unknown protein. lysozyme Chymotrypsinogen ovalbumin Serum albumin aldolase urease ferritin ovomucoid Unknown 8. Indicats M 1000 kDa 14 kDa 25 kDa 45 kDa 65 kDa 150 kDa 500 kDa 700 kDa 28 kDa ? Ve (ml) 85.00 200.00 190.00 170.00 150.00 125 90.00 92.00 160.00 130.00

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