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A series of sealed tubes containing an equal number of spores of a certain microorganism, were heated for 10 and 15 minutes at 240F (115.5C).

A series of sealed tubes containing an equal number of spores of a certain microorganism, were heated for 10 and 15 minutes at 240F (115.5C). The survivors were respectively 4600 and 160. Calculate the value of D for the microorganism

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