Answered step by step
Verified Expert Solution
Link Copied!

Question

1 Approved Answer

A solution of DNA of unknown concentration (termed [unknown] DNA solution) gave an absorbance value of 0.39 at 260nm. The goal here is to determine

image text in transcribed

A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50g/ml has an absorbance value of 1.0 at 260nm. Using this information and knowing that A1/C1=A2/C2 i. Calculate the DNA concentration (g/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many g of DNA would be present in 25 of this DNA solution iii. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260nm ) of the diluted solution be? iv. What will the new concentration of DNA of the diluted solution be

Step by Step Solution

There are 3 Steps involved in it

Step: 1

blur-text-image

Get Instant Access to Expert-Tailored Solutions

See step-by-step solutions with expert insights and AI powered tools for academic success

Step: 2

blur-text-image

Step: 3

blur-text-image

Ace Your Homework with AI

Get the answers you need in no time with our AI-driven, step-by-step assistance

Get Started

Recommended Textbook for

Organic Chemistry With Biological Applications

Authors: John E. McMurry

3rd Edition

1305367448, 978-1305367449

More Books

Students also viewed these Chemistry questions