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A solution of DNA of unknown concentration (termed [unknown] DNA solution) gave an absorbance value of 0.39 at 260nm. The goal here is to determine
A solution of DNA of unknown concentration (termed "[unknown] DNA solution") gave an absorbance value of 0.39 at 260nm. The goal here is to determine the DNA concentration of this [unknown] DNA solution. To calculate this, you are given the following additional information: A DNA solution whose concentration is 50g/ml has an absorbance value of 1.0 at 260nm. Using this information and knowing that A1/C1=A2/C2 i. Calculate the DNA concentration (g/mL) in the DNA solution of [unknown] ii. Once you have determined the DNA concentration of [unknown] DNA solution, calculate how many g of DNA would be present in 25 of this DNA solution iii. If you dilute the "[unknown] DNA solution" 10-fold, i.e. 1/10, what will the absorbance value (at 260nm ) of the diluted solution be? iv. What will the new concentration of DNA of the diluted solution be
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