A specific protein kinase (PK) gene is speculated to be differentially spliced in muscle tissue. This...
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A specific protein kinase (PK) gene is speculated to be differentially spliced in muscle tissue. This gene comprises three exons and two intron sequences and in fibroblast cells encodes a 38.5-kD protein. Investigators have transfected various portions of a genomic or cDNA copy of the PK gene (see the constructs in part (a) of the figure) into both muscle and fibroblast cells. The expression system utilizes a promoter (P in Figure a) active in both cell types, and a C-terminal fusion to a small epitope tag called V5, which contributes ~5.5 kD to the mass of the fusion protein. Part (b) of the figure shows the results of a Western blot using anti-V5 antibody, designed to analyze the expression products of the transfected cells. A negative control (Neg) of untransfected muscle cells is included. Molecular weight markers in kD are indicated on the left. (a) 1 2 3 1 1 2 = = 300 bp 3 2 VS VS 3 VS (b) 45->> 29➡>> 25-> Fibroblast Muscle Neg 1 2 3 1 2 3 What can be concluded about differential splicing of the PK gene in fibroblast versus muscle cells? O in muscle cells, the translated mRNA from construct 1 contains only exon 3. Ⓒ There is no evidence of differential splicing of construct 1 in fibroblasts and muscle cells. O In fibroblasts, the translated mRNA from construct 1 contains exons 1, 2, and 3. O in muscle cells, only exons 1 and 2 from construct 1 are included in the translated mRNA. A specific protein kinase (PK) gene is speculated to be differentially spliced in muscle tissue. This gene comprises three exons and two intron sequences and in fibroblast cells encodes a 38.5-kD protein. Investigators have transfected various portions of a genomic or cDNA copy of the PK gene (see the constructs in part (a) of the figure) into both muscle and fibroblast cells. The expression system utilizes a promoter (P in Figure a) active in both cell types, and a C-terminal fusion to a small epitope tag called V5, which contributes ~5.5 kD to the mass of the fusion protein. Part (b) of the figure shows the results of a Western blot using anti-V5 antibody, designed to analyze the expression products of the transfected cells. A negative control (Neg) of untransfected muscle cells is included. Molecular weight markers in kD are indicated on the left. (a) 1 2 3 1 1 2 = = 300 bp 3 2 VS VS 3 VS (b) 45->> 29➡>> 25-> Fibroblast Muscle Neg 1 2 3 1 2 3 What can be concluded about differential splicing of the PK gene in fibroblast versus muscle cells? O in muscle cells, the translated mRNA from construct 1 contains only exon 3. Ⓒ There is no evidence of differential splicing of construct 1 in fibroblasts and muscle cells. O In fibroblasts, the translated mRNA from construct 1 contains exons 1, 2, and 3. O in muscle cells, only exons 1 and 2 from construct 1 are included in the translated mRNA.
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The data presented indicates that the PK gene is transcribed differently in muscle and fibroblast ce... View the full answer
Related Book For
Molecular Cell Biology
ISBN: 978-1429234139
7th edition
Authors: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Matthew P. Scott
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