(a) The extraction and purification of fermentation products may be difficult and costly. Discuss how particular products can be obtained by removing unwanted materials or certain impurities to allow the products to be enriched and concentrated. (12 Marks) (b) During upstream bioprocessing, it is required to use media with high protein content containing corn-steep liquor. Discuss the problem that might occur when using that kind of media and suggest the possible solution to overcome the problem. Biotech Sdn Bhd has decided to produce an intracellular protein produced by fungi using 100L bioreactor. At the end of the process the company needs to have a target protein with high purity requirement. (a) Illustrate the general flowchart of downstream processing for bioreactor production process. Include ONE (1) suitable technique for each downstream steps given. (12 Marks) (b) Discuss the requirements needed to design stirred tank bioreactor for the product described above including the type of impeller with its flow regime, a suitable vessel and sterilization approach. Provide a schematic diagram where necessary and justify your answer accordingly