a. What are the advantages and disadvantages of internal standard calibration? In which context is it usually used? Would it make a difference in the quantification if a volume of unknown other than b. luL was injected? Consider a small difference, say 0.8uL and a large difference say 5uL. c. Discuss the different chromato and shape, etc.). Specify average retention time for all analytes What advantages are gained by using a temperature gradient during a chromatographic run (instead of keeping it constant, as you did in the lab)? What are the advantages and disadvantages of a TCD detector? Would another detector be better suited for the analytes quantified in this experiment? d. e. a. What are the advantages and disadvantages of internal standard calibration? In which context is it usually used? Would it make a difference in the quantification if a volume of unknown other than b. luL was injected? Consider a small difference, say 0.8uL and a large difference say 5uL. c. Discuss the different chromato and shape, etc.). Specify average retention time for all analytes What advantages are gained by using a temperature gradient during a chromatographic run (instead of keeping it constant, as you did in the lab)? What are the advantages and disadvantages of a TCD detector? Would another detector be better suited for the analytes quantified in this experiment? d. e