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A. What type of ionization source (e.g. CI/EI etc.) would you choose use in the following applications and why? (i) LC-MS of lipids (ii) GC-MS

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A. What type of ionization source (e.g. CI/EI etc.) would you choose use in the following applications and why? (i) LC-MS of lipids (ii) GC-MS of pharmaceuticals (iii) Imaging animal tissue sections B. Explain one way of performing tandem mass spectrometry (MS/MS) C. A Fourier Transform type mass analyser (e.g. an Orbitrap) is used to analyse the drug Amiodorone (C25H2sl2NO3) that has a monoisotopic mass of 645.023686 and has been ionised by proton transfer during electrospray ionisation. (i) A peak is observed at m/z646.03201 what is the mass accuracy of this measurement in ppm.? (ii) The peak is 0.0013m/z wide at half height, what is the "mass resolution" of the experiment? Things you might need: C=12.0000,O=15.9947,H=1.0078,N=14.003074,I=126.904477

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