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a) Yeast is to be produced in continuous culture using glucose as the limiting substrate. The chemostat has a working volume of 100m3, cell growth
a) Yeast is to be produced in continuous culture using glucose as the limiting substrate. The chemostat has a working volume of 100m3, cell growth can be adequately described by the Monod equation and the organism has the following specific characteristics: Monod's Constant (or Substrate affinity constant): Ks=0.1kgm3 Maximum specific growth rate: max0.4h1 Biomass yield on glucose: Yx/s=0.4 i) Starting from mass balances, mathematically show how the steady-state biomass [50] concentration (CX) vary in a well-mixed chemostat with single limited nutrient. Clearly state all assumptions made. ii) Calculate the steady-state substrate and biomass concentrations when the input [20] rate of feed is 20m3h1 and the glucose concentration is 40kgm3. b) Graphically show how the concentration of biomass and substrate vary with the dilution [30] rate in a CSRR. Please mark all the important regions and the values in your graph
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