aaaa Idris has successfully extracted enzymatic proteins from the fish viscera (intestines and stomach). After homogenization and centrifugation, he managed to pool the crude enzyme extract. He is characterizing the enzymes. Please help out Idris by answering the following questions he posted: (a) How do I determine the enzyme concentration? Please give its unit. (b) I intend to purify the enzymes from the crude enzyme extract. The extract contains at least five enzymes as shown in Table 1. Which chromatography method should I use to separate the enzymes based on the properties stated below (i, ii and ill)? For each, give the reasons for the choice and describe the likely chromatographic profile of the enzyme separation (Starting with the enzyme eluted first). i. Sizes ii. Charges iii. Hydrophobicity (2) iv. I am going to analyse my enzymes qualitatively by running a Sodium (2) Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). What is the principle behind this technique? Arrange the enzyme protein bands from top to bottom on the SDS- PAGE gel. Table 1 Enzymes Molecular Hydrophobicity Net charges Weight (%) at pH 7 (Da) 48.500 5 5,000 45 250,000 30 150,000 20 E 60,500 65 (c) The experiments I conducted showed that one of the enzymes was successful in producing free fatty acid from triacylglycerides. i. Which family of enzyme does this enzyme belongs to? ii. What is the possible name of this enzyme? (1) (d) How do I determine the enzyme activity? Please give the unit. (1) (e) How do I get the specific activity of this enzyme? Please give the unit. (1) (1) How do I run the experiment to find the kinetic properties of the enzyme, Km (2) and V? A B U