Q1. Perform molecular docking with your vaccine product with TLR-7 and TLR-4 receptor and find the binding affinity score to validate your vaccine model. Provide all the description from I-Tasser results as following:
1. Proteins sequence of vaccine

2. Secondary structure
3. Tertiary structure (which is more likely to be considered as final vaccine model) Enlist Ligands
5. Molecular and cellular function.
6. Molecular docking with TRL receptor.

(Toll- like receptors (TLRs) are a class of proteins that play a key role in the innate immune system).

(Note: First 24 students (068 to 162) use TLR7 and remaining from (164 to 216) use TLR4 receptor for molecular docking

Q2. Use GEO2R Genome Analyzer in the database of Gene Expression Omnibus database. Perform genome analysis e.g., smoke cancer, bone cancer, lung cancer etc. Write down it's following steps.
(Query language: osteosarcoma series dataset, smoke cancer series dataset)

Q3. Perform Phylogenetic Analysis of SARS CoV-2 genome among five Asian and five European countries. Explain in your own words, that which country has similarity and variation in the genome in written along with tree (rooted and unrooted) results. Write down its following steps.