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Aerobacter aerogenes is cultivated in a 3 m 3 bioreactor with a diameter of 1 . 5 m . A pre - aerated fresh nutrient

Aerobacter aerogenes is cultivated in a 3m3 bioreactor with a diameter of 1.5 m. A pre-aerated fresh nutrient medium containing dissolved oxygen at a concentration of 0.01 moles/m3 enters the bioreactor at a flow rate of 1.8 m3/h. Under steady-state conditions, the bioreactor operates at a 5 kg/m3 cell concentration. a. Determine the KLa required to ensure a dissolved oxygen concentration of 0.05 mole/m3 in the bioreactor. b. What minimum KLa is required if the bioreactor's critical oxygen concentration is 0.01 mole/m3? DATA: Specific oxygen uptake rate =20 moles O2/kg cellsh.
Show all the steps and calculations clearly, the ansers should match these:Start by writing mass balance for oxygen in the bioreactor. Estimate C* using Henrys law and then kLa. Answers: (a) kLa =0.136 s-1,(b)(kLa)min =0.113 s-1.

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