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After ammonium sulfate precipitation, you choose gel filtration as the next step. You reason that this will speed up the purification, because you will not
After ammonium sulfate precipitation, you choose gel filtration as the next step. You reason that this will speed up the purification, because you will not need to dialyze the protein after you have dissolved the precipitated protein in buffer; instead, you will load it on a gel filtration column. Why do you think this will work? Ammonium sulfate will elute from the column earlier than the protein because the salt is much smaller and thus moves faster. Ammonium sulfate will elute from the column later than the protein because the salt is much smaller and thus enters the pores of the gel filtration material more often. The gel filtration column will bind the ammonium sulfate via ionic interactions, while the protein passes through
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