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Although membrane filtration processes have several advantages, a thorough evalu - ation of the process parameters influencing membrane fouling is necessary to optimize permeate flow,
Although membrane filtration processes have several advantages, a thorough evalu
ation of the process parameters influencing membrane fouling is necessary to optimize
permeate flow, and ah the optimal concentration of the filtered or retained enzyme. The
use of sequential separation processes eg microfiltration and ultrafiltration can reduce
the number of downstream steps, thus minimizing production costs.
In this activity, the aim is to concentrate the lipases produced by Aspergillus niger
through sequential membrane separation processes, as shown below:
Culture Prefiltration Microfiltration Ultrafiltration concentr
ation
Based on previous laboratory pilot tests with a breeding ground of Aspergillus niger,
with an approximate concentration of it has been determined that the prefilter
is capable of removing about of the biomass, which drastically reduces the viscosity
of the medium to approximately On the other hand, the microfiltration membrane
retains the rest of the biomass as well as other smells of the micron order and
produces the following experimental data when operated at constant pressure psi:
Table : Microfiltration data for A niger breeding ground
The resulting permeate is processed by tangential flow ultrafiltration in order to be
concentrated. Based on the previous information, it answers as follows:
Make a block diagram of the lipase production and recovery process Microfiltration
Plot the change in membrane and cake resistances over time. Which of the
two resistances is more significant? Why?
Based on the operating volume you previously calculated for your problem situa
tion, estimate the filtration area needed to process the culture medium within a
maximum of hours
Ultrafiltration
Make a proposal for the molecular cutoff size that the ultrafiltration membrane
should have to retain the lipase. Justify your answer
Whereas the permeate flux of ultrafiltration is and remains rela
tively constant throughout the operation, making a proposal of the filtration area
and process time to remove of the fluid. Justify your answer
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