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An endocrine - disrupting chemical was determined by UV - Vis spectrophotometry and standard addition method. For the addition, 1 0 0 . 0 uL

An endocrine-disrupting chemical was determined by UV-Vis spectrophotometry and standard addition method. For the addition, 100.0 uL of a 1000.0 ug/mL standard was added to 100.0 mL of solution. The following data were obtained:
Absorbance of reagent blank =0.020
Absorbance of sample =0.540
Absorbance of sample plus spike =1.040
Calculate the concentration of the sample in ug/mL

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